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2003, Cilt 33, Sayı 4, Sayfa(lar) 356-360
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Detection of Brucella Antigens in Different Cheese in the Erzurum Area by ELISA
Medine Güllüce, Ahmet Adıgüzel, Ö. Faruk Algur
Atatürk Üniversitesi Fen Edebiyat Fakültesi Biyoloji Bölümü, Erzurum
Keywords: Cheese, brucellosis, brucella abortus, ELISA

In this study, the occurence of Brucella abortus antigens in different cheese samples collected from Erzurum has been investigated by using enzyme- linked immunosorbent assay (ELISA). Brucella abortus antiserum was used as antibody. Peroxsidase- labeled Protein G was used as conjugate. Hidrogen peroxidase (3%) and ABTS (2-2- azino di-3- ethyl benthiozaline- 6 sulfanate) in citric acid was used as substrate. Totally 120 white cheese, 60 civil cheese and 52 lor cheese were analyzed and 26 of totally 120 white cheese were found to be Brucella positive. In civil and lor cheese samples no antigen of Brucella was encountered.

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Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim