2010, Cilt 40, Sayı 2, Sayfa(lar) 097-108 |
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Class 1 and Class 2 Integron Gene Cassettes and Characterization of Antibiotic Resistance in Coliforms of Sea Water Origin |
Feyza Çolakoğlu1, Osman Birol Özgümüş1,2, Cemal Sandallı1, Elif Çelik Sevim1, Şengül Alpay Karaoğlu1 |
1Rize Üniversitesi Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Mikrobiyoloji ve Moleküler Biyoloji Araştırma Laboratuvarı, Rize 2Rize Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Rize |
Keywords: Sea water, coliform bacteria, class 1 and class 2 integrons |
Objective: The aim of this study was molecular characterization of the antibiotic resistance in 43 coliforms
isolated from the east coastal zone of Black Sea, Turkey.
Materials and Methods: All strains were screened for resistance to certain antibiotics by disk diffusion method.
TEM-type β-lactamase gene in ampicillin-resistant strains, class 1 and class 2 integron gene cassettes and
tetracycline resistance determinants in tetracycline-resistant strains were investigated by polymerase chain
reaction. Plasmid transformation experiments were performed by heat shock method.
Results: We found that 17 (39.5%) strains were resistant to one or more antibiotics. One ampicillin-resistant
Escherichia coli strain harbored a TEM-1 type β-lactamase gene. tet(B) gene was common among tetracyclineresistant
strains. One E. coli strain carried a 2.0-kb class 1 integron, and one E. coli carried a 2.2-kb class
2 integron. DNA sequencing results demonstrated that class 1 and class 2 integrons contained blaOXA-30-aadA1
and dfrA1-sat2-aadA gene cassette arrays, respectively. Resistance to ampicillin, tetracycline or streptomycin
was transferable traits.
Conclusion: We are of the opinion that there are antibiotic resistance determinants in coliforms of human origin
in marine environment, carrying a risk for public and environmental health. To our knowledge, this is the first
report for class 1 and class 2 integrons in coliform bacteria isolated from sea water in Turkey.
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