Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2010, Cilt 40, Sayı 3, Sayfa(lar) 131-142
[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
The Use of Fluorescence In Situ Hybridization (FISH) Method in Clinical Microbiology
Gülay Börekçi
Mersin Üniversitesi Sağlık Yüksekokulu, Mersin
Keywords: FISH, microbiology, identification

The fluorescence in situ hybridization (FISH) which is a molecular method that allows phylogenetic identification of microorganisms in mixed assemblages from direct samples without prior cultivation, is based on the use of rRNA-targeted probes and works by means of epifluorescence, confocal laser scanning microscopy or flow cytometry. The sensitivity and specificity of FISH method are very high in prenatal diagnosis, cytogenetics biotechnology, molecular ecology and medical microbiology. The application of FISH method is easy and practical the cost per patient is less than PCR and conventional culture methods and the identification time is shorter (approximately 2 and 3 hours). This review is focused on classical FISH method that is applied for the rapid identification of microorganisms and its use (principle, method, advantage and disadvantages) in clinical microbiology.

[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim