Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2012, Cilt 42, Sayı 3, Sayfa(lar) 110-114
[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
An Investigation of Legionella Species in the Water System of a University Hospital
Seyda İĞNAK, Bülent GÜRLER
İstanbul Üniversitesi İstanbul Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul
Keywords: Legionella species, water system, hospital infection

Objective: It is well known that Legionella spp. colonization of water systems of hospitals can provoke nosocomial legionellosis especially in immunocompromised patients who had undergone invasive surgery or had underlying chronic diseases. In our study, the source and the prevelance of Legionella spp. in water system of Hospitals of Istanbul Medical Faculty were investigated.

Materials and Methods: In our study, between September 2006-February 2007, 100 water samples (92 from showerheads and taps, 8 from water tank) obtained from 15 different departments and management buildings in the Hospitals of Istanbul Medical Faculty were analyzed. All samples were concentrated via filtration and decontaminated via acid exposure method. After decontamination process the samples were cultured on glycine, vancomycin, polymixin B, and cycloheximide (GVPC) Legionella selective agar. Legionella suspected colonies were studied further with latex agglutination and direct fluorescence antibody methods for identification of Legionella species and serogroups level.

Results: In our research, 7% of the samples were found positive for Legionella spp. The isolation rate of Legionella pneumophila serogroup 1, which is the main causative agent of Legionnaire disease, was found to be 3 percent. Five out of 7 strains were isolated from samples obtained from the services of Department of Children's Health, and Diseases, one strain was isolated both from the samples recruited from the Anesthesia and Reanimation Unit, and water tank. Legionella strain did not grow in cultures of the samples obtained from the services whose water supply was provided from the water tank.

Conclusion: We think that the findings of this study can provide data for risk assessment and will direct authorised people to take measures about prevention of possible hospital-acquired legionellosis.


[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim