2015, Cilt 45, Sayı 3, Sayfa(lar) 136-144 |
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Investigation of Aloysia citriodora Palau Samples in the Market in Terms of Antioxidant Capacity and Microbiological Contamination |
Ayşegül KÖROĞLU1, Nurten ALTANLAR2, Gülsen KENDİR1, Duygu ŞİMŞEK2 |
1Ankara Üniversitesi Eczacılık Fakültesi, Farmasötik Botanik Anabilim Dalı, Ankara 2Ankara Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Ankara |
Keywords: Aloysia citriodora Palau (syn. Lippia citriodora Kunth.), microbiological contamination, total phenol contents, antioxidant capacity |
Objective: The antioxidant capacity of Aloysia citriodora Palau
samples also known as “fake balm, lemon scented melissa, lemon
balm”, purchased from herbalists in different provinces of our country
was aimed to be determined. In this context, total amount of phenolic
compounds of the samples were calculated. Additionally, compliance
of the samples sold in the market, to the public health was evaluated
in terms of microbiological contamination.
Material and Method: In this study, 11 different samples were
purchased from four different provinces. Besides standard samples
were obtained from Yalova and were dried under suitable conditions.
Antioxidant capacity of the samples was examined by two different
methods: 1. Qualitative and quantitative DPPH• (2,2-diphenyl-1-
picrylhydrazyl) method for determination of free radical scavenging
capacity, 2. TBA (thiobarbituric acid) method for the determination
of liposome lipid peroxidation. Total phenol content of the samples
was also determined as gallic acid equivalent by using Folin-
Ciocalteu method. Microbiological contamination of the samples
was examined according to the European Pharmacopoeia.
Results: Total phenol content in the samples was determined as
gallic acid equivalence of 213-483.56 mg/g dry weight. In qualitative
DPPH• method, standard sample showed significant free radicalscavenging
capacity, while in quantitative DPPH• method, this
sample showed lower capacity than propyl gallate. In the TBA
method, highest lipid peroxidation capacity was determined for the
samples purchased from İstanbul market (İ1). In the studied
samples, the growth of pathogenic microorganisms was not observed.
However, number of microorganisms have been found to be higher
than the limits provided in the European Pharmacopoeia.
Conclusion: Total phenolic content and antioxidant capacity of
the drugs sold on the market vary. The samples of lemon balm
purchased in the market were found unsuitable for use on human
health because of the presence of microorganisms above the limits
accepted by European Pharmacopoeia. In this study the compliance
to European Pharmacopoeia of Aloysia citriodora Palau samples
sold in our various provinces in terms of microbiological
contamination was assessed for the first time.
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