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2016, Cilt 46, Sayı 1, Sayfa(lar) 027-032
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The Distribution of Hepatitis C Virus Genotypes in Patients With Chronic Hepatitis C Infection
Zeynep ÇİZMECİ
Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı,
Keywords: Epidemiology, HCV RNA, Hepatitis C virus, genotype

Objective: Hepatitis C Virus (HCV) genotyping is of crucial importance in the determination of treatment protocols and follow up of the clinical course. Among the major genotypes of HCV, genotype 1b which is associated with poorer prognosis and longer duration of treatment, is the most frequently seen genotype in Turkey. In this study, we aimed to determine the genotype distribution among 108 HCV RNA positive patients.

Material and Methods: Anti-HCV and quantitative HCV RNA tests were performed using microparticle EIA method (MEIA, Abbott Laboratories, Abbott Park, IL, USA) and real-time reverse transcriptase polymerase chain reaction method (Real-time PCR, Abbott Molecular, Inc., Des Plaines, IL, USA) respectively. The genotyping was performed using the base sequence analysis in ABI PRISM 310 Genetic Analyzer (Applied Biosystems, USA).

Results: Alanine aminotransferase (ALT) and HCV RNA levels of the patients were found to be 10-301 IU/L. (mean: 64.04±41.9 IU/L) and 1.100–12.473.751 IU/ml (mean: 1.665.034±2.463.931), respectively. According to the results of the genotyping, genotype 1b was determinated in 85 (79%), genotype 1a in 8 (7%), genotype 2 in 7 (6.5%), genotype 4 in 1 (1%), genotype 6 in 1 (1%) and mixed HCV genotype in 6 patients (5.5%). Evaluation of 6 patients with mixed HCV genotypes revealed the presence of genotype 1a and 1b (n=2), genotype 1a and 4 (n=1), genotype 1b and 3 (n=1), one patient had genotype 1b and 6 (n=1), and genotype 1a and 6 (n=1) in respective number of patients.

Conclusion: Genotype 1b was found to be the most prevalent genotype among our patients. The results from this preliminary data related to our hospital suggest that taking mixed HCV types in consideration would be helpful during initiation of treatment and clinical follow-up process in chronic HCV infected patients.


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