2016, Cilt 46, Sayı 1, Sayfa(lar) 027-032 |
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The Distribution of Hepatitis C Virus Genotypes in Patients With Chronic Hepatitis C Infection |
Zeynep ÇİZMECİ |
Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, |
Keywords: Epidemiology, HCV RNA, Hepatitis C virus, genotype |
Objective: Hepatitis C Virus (HCV) genotyping is of
crucial importance in the determination of treatment
protocols and follow up of the clinical course. Among the
major genotypes of HCV, genotype 1b which is associated
with poorer prognosis and longer duration of treatment, is
the most frequently seen genotype in Turkey. In this study,
we aimed to determine the genotype distribution among
108 HCV RNA positive patients.
Material and Methods: Anti-HCV and quantitative HCV
RNA tests were performed using microparticle EIA method
(MEIA, Abbott Laboratories, Abbott Park, IL, USA) and
real-time reverse transcriptase polymerase chain reaction
method (Real-time PCR, Abbott Molecular, Inc., Des
Plaines, IL, USA) respectively. The genotyping was
performed using the base sequence analysis in ABI PRISM
310 Genetic Analyzer (Applied Biosystems, USA).
Results: Alanine aminotransferase (ALT) and HCV RNA
levels of the patients were found to be 10-301 IU/L.
(mean: 64.04±41.9 IU/L) and 1.100–12.473.751 IU/ml
(mean: 1.665.034±2.463.931), respectively. According to
the results of the genotyping, genotype 1b was determinated
in 85 (79%), genotype 1a in 8 (7%), genotype 2 in 7
(6.5%), genotype 4 in 1 (1%), genotype 6 in 1 (1%) and
mixed HCV genotype in 6 patients (5.5%). Evaluation of
6 patients with mixed HCV genotypes revealed the
presence of genotype 1a and 1b (n=2), genotype 1a and 4
(n=1), genotype 1b and 3 (n=1), one patient had genotype
1b and 6 (n=1), and genotype 1a and 6 (n=1) in respective
number of patients.
Conclusion: Genotype 1b was found to be the most
prevalent genotype among our patients. The results from
this preliminary data related to our hospital suggest that
taking mixed HCV types in consideration would be helpful
during initiation of treatment and clinical follow-up process
in chronic HCV infected patients.
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