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2016, Cilt 46, Sayı 2, Sayfa(lar) 063-068 |
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Distribution and Antifungal Susceptibilities of Candida Species Isolated from Blood Cultures at Kahramanmaraş Necip Fazıl City Hospital in the Last Three Years |
Esra ÖZKAYA1, Ahmet ÇALIŞKAN2, Özlem KİRİŞCİ2, Seray TÜMER3 |
1Karadeniz Teknik Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Trabzon 2Kahramanmaraş Necip Fazıl Şehir Hastanesi, Mikrobiyoloji Laboratuvarı, Kahramanmaraş 3Adana Devlet Hastanesi, Mikrobiyoloji Laboratuvarı, Adana |
Keywords: Antifungal susceptibility, Candida species, blood culture |
Aim: Candida species are opportunistic pathogens that cause serious
infections. Recently, an increase is observed in hospital-acquired
fungal infections; and Candida species are reported to be responsible
for 5% of all nosocomial infections. Candida albicans is the most
common cause of candidemia. However, the incidence of non-albicans
Candida species has been increasing, and their antifungal
susceptibilities are different from each other. In this study investigation
of identification, and determination of antifungal susceptibility rates
among Candida species isolated from blood cultures which were sent
to our laboratory from various clinical units during January 2013-
December 2015 were aimed.
Material and Method: In the context of this study, blood culture samples collected at Kahramanmaraş Necip Fazıl City Hospital were examined. Samples were incubated using BACTEC/9050 (Becton Dickinson, Maryland, USA) automated system. For the identification and antifungal susceptibility tests of Candida species, Vitek version 2.0 (BioMérieux, France) automated system was used. Fluconazole, voriconazole, flucytosine, amphotericin B and caspofungin susceptibility categories of the isolates were determined according to the criteriae of Clinical and Laboratory Standards Institute (CLSI M27-A3). Results: During our study period, 8271 blood cultures were analysed in the laboratory. Six thousand four hundred and nineteen (77.6%) cultures resulted as negative. Candida species were isolated from 93 blood cultures of 50 patients. Candida species were isolated from blood cultures.of two patients (4.0%) in internal medicine services, and other patients (96.0%) with Candida growth were treated in the intensive care units. Candida parapsilosis constituted 62.0% of isolates. C. albicans was detected in 34.0% of the cultures at the second frequency. Candida tropicalis and Candida krusei species were cultured from only one (2.0%) specimen, each. No resistance was detected in antifungal susceptibility tests of flucytosine. The least susceptibility rate was observed among C. parapsilosis isolates for voriconazole. Ninety four percent of C. albicans isolates showed susceptibility to amphotericin B, and no resistance to the other antifungal agents tested. Conclusion: In conclusion, in our study Candida species were most frequently isolated from blood culture samples sent from the intensive care units. While the most frequent species isolated was C. parapsilosis, C. albicans was at the second frequency. Resistance to some of the antifungals tested was observed for some of the Candida species isolated. It was considered that identification and antifungal susceptibility tests are very important in the diagnosis and treatment of these infections in patients especially at intensive care units. |
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