2016, Cilt 46, Sayı 3, Sayfa(lar) 105-111 |
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Investigation of Biofilm Forming Capacity of Clinical Salmonella Isolatesunder Different Environmental Conditions |
Zaliha BALTACIOĞLU1, Fethiye Ferda YILMAZ1, Mine HOŞGÖR LİMONCU1, Şöhret AYDEMİR2, Alper TÜNGER2 |
1Ege Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı 2Ege Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı |
Keywords: Biofilm, Salmonella, ciprofloxacin |
Objective: This study aimed to investigate the antibiotic
susceptibility and biofilm forming capacity of isolates of
Salmonella spp., and the effect of different environmental
conditions and the presence of ciprofloxacin (CIP) on biofilm
forming ability.
Materials and Methods: Forty-four Salmonella spp. strains
isolated in 2012 in Ege University, Faculty of Medicine, Bacteriology
Laboratory of Department of Medical Microbiology, were included
in this research. CIP susceptibilities were determined by
microdilution method according to the CLSI recommendations and
biofilm forming capacities were examined using crystal violet by
spectrophotometric microplate method. Afterwards, the effects of
different ambient temperatures, NaCl concentrations, pH values
and presence of CIP (1/2MIC, MIC, 2MIC) on their biofilm forming
capaciies were assessed. The impact of each parameter on biofilm
formation in Salmonella strains with moderate and strong biofilm
forming capacities at standard conditions (pH 7.3- NaCl 0.5%-
37°C) were tested singly while the other two parameters were kept
at fixed levels (temperature: 4°C, 20°C, 37°C; pH: 4, 6, 7.3; NaCl:
0.5%, 4%, 6%).
Results: Under standart conditions, a total of 28 isolates of
Salmonella spp. with moderate (59%) and strong biofilm (5%)
forming capacities showed higher biofilm forming ability at
pH 6, NaCl concentration of 6%, and ambient temperature of
20°C when compared with pH 4, NaCl concentration of 4%,
and ambient temperature of 4°C. CIP resistance was found in
one Salmonella spp. isolate, and one isolate demonstrated
intermediate resistance to CIP. CIP inhibited both biofilm
formation and showed inhibitory effect on pre-formed biofilm
layer in a modarate biofilm producer.
Conclusion: This study revealed that Salmonella isolates
increased their changes of staying alive in certain conditions
by increasing their biofilm forming ability and CIP was found
to be effective on biofilm formation.
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