2019, Cilt 49, Sayı 1, Sayfa(lar) 011-016 |
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Bacterial and Viral Analysis of Cerebrospinal Fluid Samples in Patients with Suspected Encephalitis/Meningitis In a University Hospital |
Hüseyin Agah Terzi, Özlem Aydemir, Engin Karakeçe |
Sakarya Üniversitesi Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Sakarya, Türkiye |
Keywords: Acute bacterial meningitis, celebrospinal fluid, encephalitis, Herpes simplex virus |
Objective: Meningitis and encephalitis are infectious diseases with high risk of complications that
must be diagnosed and treated immediately. In this study we aimed to investigate the
bacteriological culture results of cerebrospinal fluid (CSF) samples and the frequency of Herpes
Simplex Virus (HSV-1/2) in suspected cases of viral infections of central nervous system (CNS).
Method: CSF samples of a total of 192 patients with suspected CNS infection were evaluated
retrospectively. VITEK 2 (bioMérieux, France) automated system was used for the identification
of species, and antibiotic susceptibility tests. Protein and glucose levels in CSF samples were also
recorded. Presence of HSV-1/2 DNA was qualitatively evaluated with real-time PCR technique by
using Artus® HSV ½ QS-RGQ (Qiagen, Germany) kits on Rotor-Gene system (Qiagen, Germany).
Results: Bacteria were isolated in 11 (6%) of 192 CSF samples. Isolated microorganisms were
Streptococcus pneumoniae (45%), Staphylococcus epidermidis (18%), Klebsiella pneumoniae
(9%), Citrobacter koseri (9%), Pseudomonas aeuroginosa (9%), and Listeria monocytogenes (9%).
HSV-1 DNA was detected in five (4.5%) of the 110 samples sent to our laboratory with the thought
of viral etiology. The CSF glucose levels were found to be lower and protein levels higher in 11
patients whom we obtained bacterial isolates from culture. Also in the CSF samples of the HSV-1
positive group higher glucose levels in 4, and protein levels in 3 patients were detected
Conclusion: Epidemiology of pathogens causing menengitis and encephalitis in our region will
contribute to surveillance data. Also monitoring the antibiotic susceptibilities of the isolated
bacteria regularly can contribute to improve efficient therapy strategies.
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