The aim of this study was to compare the liquid hybridisation method which is used as a standard method for detection of HBV DNA and a new method, real-time PCR. Fifty-five serum samples, received for routin screening and hepatitis B markers (HBsAg, antiHBc total) were found positive by mikro EIA (Organon Teknika), were evaluated in the study. All samples were tested with real-time PCR and liquid hybridisation method. Twenty-nine of 55 samples were found positive with both real-time PCR and liquid hybridisation method. Thirty-one of 55 samples (56%) were positive only with the liquid hybridisation, and 50 samples (90%) were positive only with the real-time PCR. In 21 samples HBV DNA were found negative with liquid hybridisation but were found positive with real-time PCR, however, 20 of this 21 sera, HBV DNA levels were lower than the 1.4x10⁶ copy, which is known the detection limit of the liquid hybridisation method. Furthermore, 2 samples which was found negative with the real-time PCR, were evaluated as positive with the liquid hybridisation method. There was no statistically meaningful difference between both methods (r=0.754, p<0.05). In conclusion, because of the real-time PCR has a broad detection interval and its sensitivity for the very low level of HBV DNA, it may be used as a sensitive and reliable method for the quantitation of HBV DNA.