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2021, Cilt 51, Sayı 2, Sayfa(lar) 109-118
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Comparison of the Antimicrobial Susceptibility Results of Bacteroides fragilis Group Isolates Determined by Agar Dilution and the Tentative EUCAST Disk Diffusion Method
Semra Eminoğlu1, Bermal Tekeş1, Elvan Sayın2, Nurver Ülger Toprak1
1Marmara Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
2Marmara Üniversitesi Pendik Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, İstanbul, Türkiye
Keywords: Bacteroides fragilis group bacteria, agar dilution method, disc diffusion method

Objective: In this study it was aimed to determine the antimicrobial susceptibility of Bacteroides fragilis group (BFG) bacteria using recently developed European Committee on Antimicrobial Susceptibility Testing (EUCAST) disc diffusion method and agar dilution method recommended by Clinical Laboratory Standart Institude (CLSI) for anaerobes and to investigate the agreement of the results of two tests.

Method: The antimicrobial susceptibilities of a total of 56 BFG strains isolated from clinical samples and identified by MALDI-TOF MS analysis between January 2017 and December 2018, were tested to ampicillin, ampicillin/sulbactam, cefoxitin, imipenem, clindamycin, tigecycline, moxifloxacin and metronidazole MICs were determined by agar dilution method using sheep blood supplemented Brucella agar and disk diffusion test using host blood supplemented Fastidius Anaerobic Agar (FAA).

Results: Six different BFG species consisting mostly strains of Bacteroides fragilis (n=34, 61%) and Bacteroides thetaiotaomicron (n=11, 20%) isolated from various clinical samples such as intraabdominal abscess (n= 24), blood (n=10) and tissue biopsy samples (n=11).were identified. Imipenem and metronidazole were the most effective antimicrobials with 98.2% susceptibility rates, followed by tigecycline, ampicillin/sulbactam, moxifloxacin and clindamycin with susceptibility rates of 89.3%, 66.1%, 57.1% and 46.4%, respectively. Most concordant results were obtained with metronidazole (100%), imipenem (89.8%) and tigecycline (89.8%). Acceptable compliance rates were not found for other antimicrobials.

Conclusion: We can say that disc diffusion method is a fast, easy-to-apply, and reliable method used in clinical microbiology laboratories to determine the susceptibility of BFG bacteria to metronidazole, imipenem and tigecycline. However, to evolve a standard method especially for other antimicrobials, the experimental conditions should be optimized with studies dome with greater number of isolates.

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