Objective: Our aim is to determine the seroprevalence of toxoplasmosis in pregnant women and the frequency of congenital toxoplasmosis in our hospital, as well as to examine the test order algorithms used for diagnosis, to question how test orders are made and how they should be, and the necessity of screening pregnant women for Toxoplasma gondii.

Methods: Toxoplasma gondii IgM, IgG, IgG avidity results and amniotic fluid PCR results of pregnant women who admitted between May 1, 2020 and May 31, 2022 were evaluated retrospectively through the review of the records of hospital information system.

Results: Of the 3555 pregnant women, 590 were IgG seropositive; 2058 were IgM and IgG negative. It was observed that the most requested test profile was the combination of IgM and IgG (73.6%). Toxoplasmosis was suspected serologically in 2.25% of the pregnant women, but it was observed that 61.25% of them were not followed up nor treated after the first test request. PCR was performed in seven pregnant women with suspected toxoplasmosis, all of which were found to be negative. It was observed that 57.9% of pregnant women were at risk of contracting toxoplasmosis during their pregnancy. Although it is not included in the Prenatal Care Management Guide, it was determined that at least one T. gondii-related test was requested in 34.88% of the pregnant women who were admitted for follow-up. No consensus was noticed among clinicians on how to perform prenatal toxoplasmosis screening, and therefore different test order algorithms were used. It was determined that 49 of 80 pregnant women with suspected toxoplasmosis were not followed up.

Conclusion: No established algorithm was seen neither for suspicious case follow-up nor for treatment planning, as in the toxoplasmosis diagnostic algorithm. However, it is critical to screen pregnant women for these preventable infections, and prenatal screening of pregnant women is highly essential. Regarding the regional toxoplasmosis seropositivity rates in our country, development of different screening policies and treatment algorithms for toxoplasmosis could end this controversial situation.