Objective: We aimed to investigate the distribution of Human papillomavirus (HPV) types, the presence of Trichomonas vaginalis in HPV DNA-positive patients, and metronidazole resistance in HPV DNA/T. vaginalispositive samples using molecular methods.

Methods: A total of 200 patient samples, including 100 cervical samples with positive and 100 samples with negative results in the Microbiology laboratory, who applied to Manisa Celal Bayar University, Department of Gynecology and Obstetrics, were routinely asked for an HPV test, included in the study. These remaining samples were taken after the HPV DNA analysis scanned for the presence of T. vaginalis in the Parasitology laboratory, and the samples were investigated for metronidazole resistance with ntr4 and ntr6 primers that we designed specific to the T. vaginalis nitroreductase gene region.

Results: Of the 100 HPV DNA positive samples, 20 were typed as HPV 16, 8 as HPV 18, 64 as HR HPV, 6 as HPV 16/HR HPV and 2 as HPV 18/HR HPV. T. vaginalis DNA positivity was detected in one of the patients with HPV DNA positivity and typed as HR-HPV. T. vaginalis DNA was not detected in any of the HPV DNA negative patients. As a result of the PCR, ntr4 mutation was detected in 1 sample in which the presence of T. vaginalis DNA was detected, while ntr6 mutation was not detected.

Conclusion: Preventing HPV infection, screening for sexually transmitted diseases such as T. vaginalis without ignoring coexistence are important for the reduction of the rates of sexually transmitted diseases with early and rapid treatment.