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2002, Cilt 32, Sayı 3-4, Sayfa(lar) 286-289
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Extraction of Aspergillus niger van Tieghem, an Allergenic Microfungus, and Application of Toxicity Test
Günay Çolakoğlu
Marmara Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Mikrobiyoloji Bilim Dalı, İstanbul, Türkiye
Keywords: Allergy, A.niger extract, toxicity test
Abstract
In this study, for the first time in Turkey, Aspergillus niger van Tieghem, isolated from the soil of Belgrad Forest, was extracted and some toxicity tests were carried out in order to determine its toxic effects on mice. The method of extraction complied with the one described in the literature. During the process Coca's solution was used as an extractive agent. Sterile filtration technique was employed in the sterilization process. The stock solution was diluted to 1/10 of its concentration before the application of toxicity tests. It was made certain that the A.niger van Tieghem extract was sterile and that, as the experiments made on 10 mice proved, it was not toxic.
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