2006, Cilt 36, Sayı 4, Sayfa(lar) 184-189 |
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Hepatic damage due to T cell activation: An experimental model |
Nuray Gürel-Polat1, Suzan Adın-Çınar2, Aydın Çevik2, Mutlu Küçük2, Selim Badur1 |
1İstanbul Üniversitesi İstanbul Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji ABD Viroloji ve Temel İmmünoloji BD, İstanbul 2İstanbul Üniversitesi Deneysel Tıp Araştırma Enstitüsü, İstanbul |
Keywords: Balb/c mice, Con-A, LPS, liver damage, peripheral lymphocyte subgroups |
This study attemps to have an insight into the immunopathogenesis of viral hepatitides, which are a major health problem throughout the whole world. For this purpose, hepatitis has intentionally been produced in laboratory animals by injecting them mitogenic or toxic substances know to have an immunologic effect the immune system.
In this study, development of hepatic damage due to T. cell activation was investigated in vitro. By injecting ıv 10μg/ml endogenous mediator lipopolysaccharide ( LPS ), acute inflammatory liver disease was produced in BALB/c mice. Likewise, autoimmune chronic active hepatitis was observed to develop in the mice in which Con-A of the lectins was used ( ıv 0,3 mg/mice ). Acute hepatic damage was evidenced by the increase of the transaminase levels followed up for 24h. A flow cytometer was used in measuring the peripheral blood lymphocyte subgroups of CD4, CD8, CD3, NK and CD25. In the Con-A and LPS groups, a significant increase in the expression of NK ( p<0,01; p<0,005 respectively ) and CD25 ( p<0,003; p<0,0001 respectively ) was observed. The histopathological examination of the liver tissues of both the control and experiment groups revealed that in the latter group, there was hepatic damage around the central veins and portal areas due to mononuclear and polymorphonuclear cell infiltration.
The results show that the experimental method employed in this study will make it possible to investigate cells that are responsible for the development of hepatitis due to T cell activation, various cytokines and apoptosis .
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