| 2003, Cilt 33, Sayı 3, Sayfa(lar) 266-268 |
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| Comparison of Enzyzme Immunoassay ( EIA) and COBAS AMPLICOR HCV- RNA Test Results in Patiens with suspected Hepatitis C Infection |
| Banu Bayraktar1, Ayfer Eren Şensoy2, Emin Bulut1, Alper Gündüz2, Engin Seber2 |
1 Şişli Etfal Eğitim ve Araştırma Hastanesi Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İstanbul
2 Şişli Etfal Eğitim ve Araştırma Hastanesi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, İstanbul |
| Keywords: Diagnosis of HCV infection, HCV RNA, PCR, Serology |
Serum samples obtained from 95 patients with suspected HCV infection were analyzed. 73 anti-HCV positive, 22 anti –HCV negative samples were examined with PCR for the absence or present of viremia. Serology and PCR methods gave concordant results for 75 samples, 58 of them were positive and 17 of them were negative in both assays. In 14 anti- HCV positive cases HCV- RNA was detected negative . A negative result in the HCV- EIA was observed in two HCV- RNA positive samples. When compared to the patients serological status, as assesed by anti-HCV EIA, the results of PCR showed an overall concordance rate of 82,4%.
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