| 2007, Cilt 37, Sayı 4, Sayfa(lar) 204-208 |
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| Usage of polymerase chain reaction in the diagnosis of tuberculous meningitis |
| Gönül Aslan1, Gürol Emekdaş1, Mahmut Ülger1, Seda Tezcan1, Ali Kaya2, Okan Doğu3, Necdet Kuyucu4 |
1Mersin Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, Mersin
2Mersin Üniversitesi Tıp Fakültesi Klinik Mikrobiyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı, Mersin
3Mersin Üniversitesi Tıp Fakültesi Nöroloji Anabilim Dalı, Mersin
4Mersin Üniversitesi Tıp Fakültesi Çocuk İnfeksiyon Hastalıkları Bilim Dalı, Mersin |
| Keywords: Tuberculous meningitis, early diagnosis, polymerase chain reaction |
The early diagnosis of tuberculous meningitis (TBM) is very important. In this study, the value of the polymerase chain
reaction (PCR), one of the DNA-based assays, was compared together with conventional methods [Ehrlich-Ziehl-Neelsen
(EZN) and culture] for the detection of Mycobacterium tuberculosis in cerebrospinal fluid (CSF) from patients suspected of
TBM. Of the 68 CSF specimens from probable TBM patients, 7 were positive by PCR, 5 were positive by culture (BACTEC
460/ Löwenstein-Jensen). No positive results were found by EZN. The PCR for this study had a sensitivity of 80%, a specificity
of 95%, a positive predictive value of 57%, and negative predictive value of 98%. In the diagnosis of TBM, PCR should be
used together with conventional culture methods.
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