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2008, Cilt 38, Sayı 1, Sayfa(lar) 027-32
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Investigation of slime production and hemolytic activity in Candida strains isolated from various clinical samples
Fahriye Ekşi, Ebru Sözen, Ayşen Bayram, Tekin Karslıgil, İclal Balcı
Gaziantep Üniversitesi Tıp Fakültesi Mikrobiyoloji Anabilim Dalı
Keywords: Candida spp, hemolytic activity, slime production.

In last years Candida species emerge as pathogens frequently isolated from several infections, especially from nosocomially acquired ones. The aim of this study was to investigate the virulence factors of Candida spp. such as slime production and in vitro hemolytic activity, which were isolated from various clinical samples sent to our laboratory. In this study 88 Candida isolates were investigated and they were typed with germ tube test, chlamydospore production, and with the identification kit of API ID 32 C (bioMerieux, France). Sixty-two of the Candida isolates were identified as C. albicans, and 8 isolates as non- C. albicans. Slime production was observed in 39 (44.3%) isolates, among which 8 (20.5%) were strongly positive, 13 (33.3%) were moderately positive, and 18 (46.2%) were weakly positive. Candida spp. did not show hemolysis in human and/or sheep blood agar after 24 and 48 hours incubation. Following incubation for 48 hours on SDA glucose agar with sheep and/or human blood, 37 (42.1%) isolates produced beta-hemolysis, 23 (26.1%) alpha-hemolysis, and 28 (31.8%) gammahemolysis.

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