Chromatographically analyzes cellular fatty acids and compares the results with the fatty acid profiles in its database. Candida strains were identified as and included Candida albicans (n=74), Candida tropicalis (n=28), Candida glabrata (n=7), Candida parapsilosis (n=3), Candida krusei (n=4) and Candida guilliermondii (n=2). In preparation for fatty acid analysis, yeast isolates were subcultured onto sabouraud dextrose agar and were incubated at 28 °C for 24h. The colonies of yeast were saponified, methylated, extracted, and chromatographically analyzed according manufacturer's instructions. Of the 118 isolates tested, 79 (66,9%) were correctly identified to the species level, with 27 ( 22,8%) being incorrectly identified and 12 (10,1%) giving no identification.