During the course of adenovirus infection, a number of viral proteins stimulate or inhibit programmed cell death ( PCD ). In this study, adenovirus was isolated from stool sample of a child. Adenoviral antigens which were determined with Latex Agglutination (LA) test were propagated in HEp-2 cell lines. Adenovirus inoculated in cell culture was confirmed by direct immunofluorescent assay (DFA). Programmed cell death (PCD) determinations were made by nuclear staining with DAPI according to morphological criteria. Cytopathogenic effects of adenovirus were followed for 36 hours of post infection (h.p.i). Determination of da DAPI within 24 h.p.i. showed that PCD in HEp - 2 cells was induced by adenovirus isolate .