Objective: The aim of this study was to investigate the antimicrobial resistance and metallo-beta-lactamase production rates of Pseudomonas aeruginosa strains isolated from patients hospitalized at intensive care unit of Çanakkale State Hospital between January 2008-January 2009.

Materials and Methods: The antimicrobial susceptibilities of 108 P. aeruginosa strains were evaluated prospectively according to CLSI M100-S18 guidelines and metallo-beta-lactamase production was investigated by double-disk synergy method .

Results: Imipenem resistance was found in 20 (18.5%) of the strains and 14 (70%) of imipenem resistant strains were phenotypically metallo-beta-lactamase positive.

Conclusion: These results indicated high rate of phenotypic metallo-beta-lactamase positivity in carbapenem resistant P. aeruginosa strains isolated in the ICU. However, this phenotypic metallo-beta-lactamase production should further be investigated to enlighten the genetic basis of carbapenem resistance in these nosocomial P.aeruginosa isolates.