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2012, Cilt 42, Sayı 4, Sayfa(lar) 142-147
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Comparison of Various Serological Methods Used for Laboratory Diagnosis of Brucellosis
Esra GÜLTEKİN1, M. Hamidullah UYANIK1, Ayşe ALBAYRAK2, Osman AKSOY2, Ahmet AYYILDIZ1
1Atatürk Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, Erzurum
Keywords: Brucellosis, ELISA, serological tests

Objective: The aim of this study was to compare the results of various serological methods in patients who were suspected to have brucellosis.

Materials and Methods: Serological techniques, including the Rose Bengal test (RBT), Standard tube agglutination test (STA), Coombs test (CT) and Indirect Enzyme-Linked Immusorbent Assay (ELISA; IgG and IgM) method were performed on patients who were suspected to have brucellosis. The sensitivity and specificity of the used methods were estimated based ELISA (IgG and/or IgM) results.

Results: 117 patients who were suspected to have brucellosis were included in the study. Eighty-five patients (57 [67.1%] males, and 28 [32.9%] females) were serologically positive as assessed by at least one serologic method of laboratory diagnosis of brucellosis. The ages of the patients ranged from 5 to 75 years old (mean, 34 years old). The positivity rates of RBT, STA, CT, and ELISA (IgG and IgM) test were 95.3%, 62.3%, 75.3%, and 78.8% respectively. When ELISA (IgG and/or IgM) positivity accepted as the reference test, sensitivities and specificities of the tests were estimated as follows; 94% and 64% for Rose Bengal, 77,6% and 98% for STA, 91% and 94% for CT, 86,6% and 100% for ELISA IgG, and 70,1% and 100% for ELISA IgM.

Conclusion: RBT and STA is not enough for all cases, to determine the clinical situation of the disease and to prevent cross reaction, non-aglutine antibody and prezone phenomena which give faulty results in the diagnosis of brucellosis. In this condition, for low titer of STA, detection of the seroconversion or some serological tests (CT and ELISA) should be used together to increase the chance of laboratory diagnosis of brucellosis.


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