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2015, Cilt 45, Sayı 1, Sayfa(lar) 041-047
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Investigating of Candida parapsilosis Strains Isolated from Blood Cultures of Patients in Intensive Care Unit in Terms of Mini-Epidemics
Efdal OKTAY, Harun GÜLBUDAK, Didem ÖZGÜR, Feza OTAĞ
Mersin Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Mersin
Keywords: Nosocomial fungal infection, Candida parapsilosis, rep-PCR DiversiLab®

Objective: Bloodstream infections are seen the most common fungal infections which is caused by catheterization in the hospitalized patients in intensive care units. Candida albicans is the first nosocomial fungal infection owing to endogenous origin. Incidence of non-albicans Candida species (Candida tropicalis, Candida parapsilosis, Candida glabrata) that have antifungal treatment problems, are increasing rapidly. The aim of this study was to determine the clonal relationship between strains of C. parapsilosis by using rep-PCR (Repetitive Extragenic Palindromic Element-Polymerase Chain Reaction, bioMeriéux, Fransa) DiversiLab® method.

Materials and Methods: A total of 33 C. parapsilosis strains isolated from blood samples obtained from central venous catheter between January 2010-June 2013 were included in the study. Clonal relationships of Candida parapsilosis strains which were type diagnosed by the conventional methods were determined by rep-PCR DiversiLab® system.

Results: Rep-PCR DiversiLab® analysis have shown the presence of three clones (A-C). Clone A was found to be the dominant type. Twenty nine (87.8%) of the 33 C. parapsilosis strains belonged to clone A, 3 (9.0%) to clone B, one strains to clone C. Clone A was isolated from 48.2%, 20.6%, 17.2%, 13.7% of the samples sent from reanimation unit, pediatric intensive care unit, surgical intensive care unit, internal diseases intensive care units, respectively. Eight month interval has been found between first and last isolations.

Conclusion: As a result; it is contemplated that C. parapsilosis strains were scattered as a result of cross transmission and patient transfer among clinics. The rep- PCR DiversiLab® system which was used in this study has been evaluated as a rapid, easily applicable and successful procedure for epidemiological studies. Clonal distribution of strains in the hospital environment emphasizes the significance of infection control measures.


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