2015, Cilt 45, Sayı 1, Sayfa(lar) 041-047 |
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Investigating of Candida parapsilosis Strains Isolated from Blood Cultures of Patients in Intensive Care Unit in Terms of Mini-Epidemics |
Efdal OKTAY, Harun GÜLBUDAK, Didem ÖZGÜR, Feza OTAĞ |
Mersin Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Mersin |
Keywords: Nosocomial fungal infection, Candida parapsilosis, rep-PCR DiversiLab® |
Objective: Bloodstream infections are seen the most common
fungal infections which is caused by catheterization in the
hospitalized patients in intensive care units. Candida
albicans is the first nosocomial fungal infection owing to
endogenous origin. Incidence of non-albicans Candida
species (Candida tropicalis, Candida parapsilosis, Candida
glabrata) that have antifungal treatment problems, are
increasing rapidly. The aim of this study was to determine
the clonal relationship between strains of C. parapsilosis
by using rep-PCR (Repetitive Extragenic Palindromic
Element-Polymerase Chain Reaction, bioMeriéux, Fransa)
DiversiLab ® method.
Materials and Methods: A total of 33 C. parapsilosis strains
isolated from blood samples obtained from central venous
catheter between January 2010-June 2013 were included in
the study. Clonal relationships of Candida parapsilosis strains
which were type diagnosed by the conventional methods
were determined by rep-PCR DiversiLab® system.
Results: Rep-PCR DiversiLab® analysis have shown the
presence of three clones (A-C). Clone A was found to be the
dominant type. Twenty nine (87.8%) of the 33 C. parapsilosis
strains belonged to clone A, 3 (9.0%) to clone B, one strains
to clone C. Clone A was isolated from 48.2%, 20.6%, 17.2%,
13.7% of the samples sent from reanimation unit, pediatric
intensive care unit, surgical intensive care unit, internal
diseases intensive care units, respectively. Eight month
interval has been found between first and last isolations.
Conclusion: As a result; it is contemplated that
C. parapsilosis strains were scattered as a result of cross
transmission and patient transfer among clinics. The rep-
PCR DiversiLab® system which was used in this study has
been evaluated as a rapid, easily applicable and successful
procedure for epidemiological studies. Clonal distribution
of strains in the hospital environment emphasizes the
significance of infection control measures.
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