2015, Cilt 45, Sayı 1, Sayfa(lar) 048-054 |
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Determination of the Microorganisms Isolated from Blood Cultures and Their Antibiotic Susceptibility Rates |
Halil ER1, Gülşah AŞIK2, Özlem YOLDAŞ2, Cengiz DEMİR2, Recep KEŞLİ2 |
1Muş Devlet Hastanesi, Mikrobiyoloji Laboratuvarı, Muş 2Afyon Kocatepe Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Afyon |
Keywords: Antibiotic susceptibility, blood culture, sepsis |
Aim: Sepsis is a serious, life-threatening condition that progresses
very quickly due to the spread of microorganisms and their toxins
in the blood. The aim of this study was to determine the distribution
and antibiotic susceptibilities of the microorganisms isolated from
blood cultures at Afyon Kocatepe University Medical Faculty
Hospital, Turkey.
Materials and Methods: Blood culture samples were incubated in
BACT/ALERT 3D automated system (Biomerieux, Marcy L'Etoile,
France) for 5 days. Identification of the microorganisms was
performed by conventional methods and the BD Phoenix
automated system (Becton Dickinson, Sparks, Maryland,
USA). Antibiotic susceptibilities were determined by the Kirby-Bauer
disk diffusion method in accordance with CLSI recommendations.
Results: Among the 4262 blood cultures, 765 (17.9%) revealed
growth of microorganisms considered as causative agents of
bacteremia and sepsis. The most frequently isolated microorganism
was Staphylococcus aureus (n:293, 38.3%) followed by coagulase
negative staphylococci (CNS) (n:139, 18.2%), Escherichia coli
(n:93, 12.1%), Enterococcus spp. (n:56, 7.3%), Klebsiella pneumoniae
(n:54, 7.1%), Acinetobacter baumannii (n:37, 4.8%), Pseudomonas
aeruginosa (n:31, 4.1%), Brucella spp. (n:25, 3.3%), Candida spp.
(n:25, 3.3%), Streptococcus pneumoniae (n:7, 0.9%) and Serratia
marcescens (n:5, 0.6%). Methicillin resistance was determined in
71.7% of S. aureus and 59.0% of CNS strains. The rate of
vancomycin resistance in enterococci was 5.4%. The highest rates
of resistance in Enterobacteriaceae were against ceftazidime.
Carbapenems were determined to be the most effective antibiotic
group against Enterobacteriaceae. Aminoglycosides were the most
effective group of antibiotics in nonfermentative bacteria.
Conclusion: The distribution of the microorganisms isolated from
blood cultures and their antibiotic susceptibility profiles vary in
each hospital and geographical area. Thus, periodical report of
antibiotic susceptibility profiles of blood pathogens is of crucial
importance for the determination of empirical treatment
protocols.
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