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2015, Cilt 45, Sayı 1, Sayfa(lar) 048-054
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Determination of the Microorganisms Isolated from Blood Cultures and Their Antibiotic Susceptibility Rates
Halil ER1, Gülşah AŞIK2, Özlem YOLDAŞ2, Cengiz DEMİR2, Recep KEŞLİ2
1Muş Devlet Hastanesi, Mikrobiyoloji Laboratuvarı, Muş
2Afyon Kocatepe Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Afyon
Keywords: Antibiotic susceptibility, blood culture, sepsis

Aim: Sepsis is a serious, life-threatening condition that progresses very quickly due to the spread of microorganisms and their toxins in the blood. The aim of this study was to determine the distribution and antibiotic susceptibilities of the microorganisms isolated from blood cultures at Afyon Kocatepe University Medical Faculty Hospital, Turkey.

Materials and Methods: Blood culture samples were incubated in BACT/ALERT 3D automated system (Biomerieux, Marcy L'Etoile, France) for 5 days. Identification of the microorganisms was performed by conventional methods and the BD Phoenix automated system (Becton Dickinson, Sparks, Maryland, USA). Antibiotic susceptibilities were determined by the Kirby-Bauer disk diffusion method in accordance with CLSI recommendations.

Results: Among the 4262 blood cultures, 765 (17.9%) revealed growth of microorganisms considered as causative agents of bacteremia and sepsis. The most frequently isolated microorganism was Staphylococcus aureus (n:293, 38.3%) followed by coagulase negative staphylococci (CNS) (n:139, 18.2%), Escherichia coli (n:93, 12.1%), Enterococcus spp. (n:56, 7.3%), Klebsiella pneumoniae (n:54, 7.1%), Acinetobacter baumannii (n:37, 4.8%), Pseudomonas aeruginosa (n:31, 4.1%), Brucella spp. (n:25, 3.3%), Candida spp. (n:25, 3.3%), Streptococcus pneumoniae (n:7, 0.9%) and Serratia marcescens (n:5, 0.6%). Methicillin resistance was determined in 71.7% of S. aureus and 59.0% of CNS strains. The rate of vancomycin resistance in enterococci was 5.4%. The highest rates of resistance in Enterobacteriaceae were against ceftazidime. Carbapenems were determined to be the most effective antibiotic group against Enterobacteriaceae. Aminoglycosides were the most effective group of antibiotics in nonfermentative bacteria.

Conclusion: The distribution of the microorganisms isolated from blood cultures and their antibiotic susceptibility profiles vary in each hospital and geographical area. Thus, periodical report of antibiotic susceptibility profiles of blood pathogens is of crucial importance for the determination of empirical treatment protocols.


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