2017, Cilt 47, Sayı 3, Sayfa(lar) 106-113 |
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The Effect of Curcumin on Several Virulance Factors of Pseudomonas aeruginosa Isolates Obtained From Respiratory Tract Samples of Cystic Fibrosis Patients |
Reyhan MUTLU, Aynur EREN TOPKAYA |
Namık Kemal Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Tekirdağ |
Keywords: Pseudomonas aeruginosa, cystic fibrosis, curcumin |
Objective: Pseudomonas aeruginosa causes respiratory tract
infections that are difficult to treat in patients with cystic fibrosis
because of the antibiotic resistance mechanisms and virulence
factors. Curcumin has been found to possess antibacterial as well
as antitumoral and anti-inflammatory effects. The aim of this study
was to investigate the effect of curcumin on several virulence
factors (biofilm, elastase, alkaline protease) of P. aeruginosa
isolates which were obtained from respiratory tract samples of
cystic fibrosis patients.
Material and Methods: In our study, minimal inhibitory
concentration (MIC) values of curcumin for P. aeruginosa isolates
(n=65) were determined by agar dilution method. Production of
three different virulence factors (elastase, alkaline protease, and
biofilm) were investigated by semi-quantitative methods which
relied on the measurement of absorbance in microtiter plates. The
isolates which had a positive virulence factor production were
retested for the production of virulence factors at the presence and
absence of sub-MIC curcumin concentrations simultaneously and
measured absorbance values were compared statistically.
Results: In our study, curcumin MICs of the 11 (16%) isolates were
250-500 μg/ml 501-1000 μg/m for 7 (11%), 1001-1500 μg/ml for
5 (8%), and above 1500 μg/ml for 42 (65%) isolates. Out of 65
isolates in 54% biofilm, in 74% elastase, and in 68% alkaline
protease production were positive. In terms of virulence factor
production, results didnt indicate a statistically significant
difference between the absorbance values obtained with and
without exposure to sub-MIC concentrations of curcumin.
Conclusion: In conclusion, there was no statistically significant
decrease in the virulence factors of cystic fibrosis isolates which
were once exposed to in vitro curcumin. However, because
curcumin is a non-toxic, anti-inflammatory and anti-bacterial
agent, it will be suitable to investigate the in vivo effects of longterm
curcumin use in these patient groups.
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