2017, Cilt 47, Sayı 3, Sayfa(lar) 131-137 |
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Investigation of the Causative Agents of Meningitis By Real-Time PCR Method |
Oya AKKAYA1, Hülya İren GÜVENÇ2, Asuman GÜZELANT1, Meral KAYA1, Şerife YÜKSEKKAYA1, Ayşegül OPUŞ1, Muhammet Güzel KURTOĞLU1, Habibe ÖVET1 |
1Sağlık Bilimleri Üniversitesi, Konya Eğitim Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, Konya 2Batman Devlet Hastanesi, Mikrobiyoloji Laboratuvarı, Batman |
Keywords: Cerebrospinal fluid, polymerase chain reaction, central nervous system infections |
Objective: Central nervous system (CNS) infections require
rapid diagnosis because of their rapid progression to death
or severe permanent sequelae. The aim of this study is to
demonstrate retrospectively, the distribution of viral and
bacterial agents detected by multiplex real-time PCR
method in CSF samples in terms of patients age and
season of the infection.
Material and Methods: CSF samples of 200 patients which
were sent to the molecular microbiology unit of our hospital
with suspect meningitis were investigated in this study.
Specimens were tested using multiplex real-time PCR assay
for adenovirus (AdV), cytomegalovirus (CMV), enteroviruses
(EV), Epstein- Barr virus (EBV), herpes simplex virus 1 and
2 (HSV), human herpes virus 6 and 7, human parechovirus,
parvovirus B19, varicella-zoster virus (VZV) and among the
bacterial pathogens Haemophilus influenzae, Neisseria
meningitidis and Streptococcus pneumoniae.
Results: A bacterial or a viral agent was detected in 50
(25%) of the 200 samples analyzed in the study. Among
positive samples most frequently (6.5%) enterovirus was
detected, followed by adenovirus and HSV-1 (5%), and S.
pneumoniae (3.5%). The highest rate of positivity was
detected within age bracket of 0-5 years. Bacterial positivity
was detected in 9 patients. The two viral coinfections were
seen in 2 (1%) patients.
Conclusion: Early diagnosis and treatment of meningitis
are very important to reduce mortality and morbidity. Early
detection of the responsible agents may be possible with
molecular methods, such as PCR. Multiplex PCR method is
an easy and rapid test and it is considered that as the
relevant studies increase in number, it will enrich the
epidemiologically valuable data.
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