2021, Cilt 51, Sayı 2, Sayfa(lar) 156-162 |
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Characterization of Phage Obtained from Methicillin -Resistant Staphylococcus aureus (MRSA) |
Özlem Ulusan Bağcı, Fikret Şahin, Mehmet Kıyan |
Ankara Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye |
Keywords: Bacteriophage, phage therapy, Staphylococcus |
Objective: The emergence of Staphylococcus aureus strains resistant to all antimicrobials and
failure to discover new antibiotics have led researchers to phage therapy, which lost popularity
after the discovery of antibiotics. The development of recombinant technology introduced the
idea of creating lysogenic recombinant phages that provide controlled bacterial death and this
required small- sized phages that were easy to manipulate. Our aim is to identify small-sized
lysogenic bacteriophages that can be used safely in therapy.
Method: The gene and protein map of the phage was created by analysis of sequencing after
extracting a phage from the MRSA strain that is known to contain a small phage.
Results: The phage was classified in Caudovirales spp. as it contains genes encoding tail proteins,
and in Podoviridae spp. due to its genomic size and arrangement.
Conclusion: To date, there are only sixteen phages from Podoviridae family uploaded on NCBI,
and the phage described in this study is the seventeenth one. Only 41.4% of the ORFs (Open
Reading Frames) in the genome could be matched with proteins using the NCBI BLAST. Recent
studies suggest that 50-75% of bacteriophage ORFs do not correspond to any organism in
GenBank. For better understanding of bacteriophages and their utilization in phage therapy, it is
essential to sequence greater number of phages, and to discover their genomes and corresponding
proteins. Since the genes and proteins of a lysogenic phage that can be used safely in recombinant
phage therapies have been identified in our study, it will contribute to the relevant literature.
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